Part:BBa_K676778:Design Deleted


Extractery


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3932
    Illegal PstI site found at 1424
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1247
    Illegal NheI site found at 3558
    Illegal PstI site found at 1424
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 867
    Illegal BglII site found at 2329
    Illegal BglII site found at 4307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3932
    Illegal PstI site found at 1424
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3932
    Illegal PstI site found at 1424
    Illegal NgoMIV site found at 1033
    Illegal AgeI site found at 2975
    Illegal AgeI site found at 3045
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1520


Design Notes

GyrB has a EcoR1 restriction site at 6th bp of the ORF, and it has been removed site directed mutagenesis. The wild type Gyr A ORF has a Pst 1 restriction site at the 1212th bp position so site directed mutagenesis was carried out to remove the restriction site.


Source

E.coli genome

References